Analysis of hydroxy fatty acids by gas-liquid chromatography |
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| Affiliation: | 1. Department of Biochemistry, City of Hope Medical Center, Duarie, California, USA;2. The Department of Pathology, University of Southern California School of Medicine, Los Angeles, California, USA;3. The Department of Medicine, University of Southern California School of Medicine, Los Angeles, California, USA;4. The Los Angeles County Hospital, Los Angeles, California, USA;1. Department of Food Environmental and Nutritional Science (DeFENS), University of Milan, Via Mangiagalli, 20133 Milan, Italy;2. Department of Pharmaceutical Sciences (DISFARM), University of Milan, Via Mangiagalli 25, 20133 Milan, Italy;3. Instituto de Catálisis y Petroleoquímica, CSIC, 28049 Madrid, Spain;1. Department of Pharmacy Practice, Jerry H. Hodge School of Pharmacy, Texas Tech University Health Sciences Center, Dallas, TX 75235, USA;2. Clinical Pharmacology and Experimental Therapeutics Center, Jerry H. Hodge School of Pharmacy, Texas Tech University Health Sciences Center, Dallas, TX 75235, United States;3. Rare Brain Disorders Program, UT Southwestern Medical Center, Dallas, TX 75390, United States;4. Department of Neurology, UT Southwestern Medical Center, Dallas, TX 75390, United States;5. Departments of Physiology, and Pediatrics, UT Southwestern Medical Center, Dallas, TX 75390, United States;6. Eugene McDermott Center for Human Growth & Development / Center for Human Genetics, UT Southwestern Medical Center, Dallas, TX 75390, United States;7. Department of Pharmaceutical Sciences, Jerry H. Hodge School of Pharmacy, Texas Tech University Health Sciences Center, Dallas, TX 75235, United States;1. Laboratório de Biologia Teórica e de Sistemas, Instituto de Bioquímica Médica Leopoldo de Meis, Universidade Federal do Rio de Janeiro, Brazil;2. Network of Researchers on Horizontal Gene Transfer and the Last Universal Common Ancestor (NoR HGT & LUCA), Leeds, UK;3. Laboratório de Genética Evolutiva Paulo Leminsk, Departamento de Biologia Molecular, Universidade Federal da Paraíba, João Pessoa, Paraíba, Brazil;1. State Key Laboratory for Advanced Metals and Materials, University of Science and Technology Beijing, Beijing, 100083, China;2. Collaborative Innovation Center of Steel Technology, University of Science and Technology Beijing, Beijing, 100083, China |
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| Abstract: | Techniques for the gas-liquid chromatographic (GLC) separation of hydroxy fatty acids are described. Procedures are given for GLC of the methyl esters of hydroxy fatty acids from 14–18 carbons, and the acetoxy fatty acid methyl esters from 14–26 carbons. The degree of unsaturation of α-hydroxy fatty acids can be determined by GLC of acetoxy fatty esters on polyester columns. The position of the hydroxyl group on the carbon chain markedly affects retention time, as shown by GLC of hydroxymethylpalmitates with the hydroxyl group in positions 2–16. Procedures are described for the rapid preparation, characterization, and quantitation of saturated and unsaturated 2-acetoxy fatty acid methyl esters ranging from 14–26 carbons encountered in sphingolipids. |
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