Quantitative analysis of carbohydrates using gas-liquid chromatography |
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| Affiliation: | 1. University of Sfax Tunisia, Faculty of Sciences, Department of life sciences, Laboratory of Biodiversity and Aquatic Ecosystems, Ecology and Planktology, Unit UR 11 ES 72/Street of Soukra Km 3,5,B.P. 1171, CP 3000, Tunisia;2. University of Sfax Tunisia, Faculty of Sciences, Department of life sciences, Laboratory of Animal Ecophysiology, B.P. 95, 3000, Tunisia;1. Unit of Forensic Medicine, Dept. of Diagnostics and Public Health, University of Verona, Verona, Italy;2. Institute of Translational Medicine and Biotechnology, Sechenov First Moscow State Medical University, Moscow, Russia;1. Department of Pharmacognosy, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi Arabia;2. Department. of Botany and Microbiology, College of Science, King Saud University P.O. Box 2455, Riyadh 11451, Saudi Arabia;3. National Center for Natural Products Research, School of Pharmacy, University of Mississippi, Mississippi, MS 38677, USA;4. Department of Biomolecular Science, School of Pharmacy, University of Mississippi, Mississippi, MS 38677, USA |
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| Abstract: | Sugars can be analyzed quantitatively as their trimethylsilyl derivatives using gas-liquid chromatography. Standardization of polyethylene glycol succinate columns with α-methylgalactopyranoside as the standard gave a quantitative correlation between GLC relative peak areas and relative molar compositions of sugar standard solutions for: the pentose, ribose; the deoxyhexose, fucose; and the hexoses, glucose, galactose, and mannose. Quantitative data were also obtained for N-acetylamino sugars. Mixtures of sugars were analyzed by using the standardization graphs, and the amounts determined were accurate within 10%. This use of GLC will make it easier for the biochemist to attack problems in carbohydrate chemistry by enabling quantitative work to be done readily on mixtures heretofore difficult to analyze. |
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