Effect of thapsigargin on inhibitory synaptic transmission between cultured neurons of the rat hippocampus

We carried out electrophysiological experiments on cultured neurons of the rat hippocampus. The voltage-clamp technique and extracellular stimulation of single presynaptic axons were used for measurements of the evoked inhibitory postsynaptic currents (eIPSCs). It was found that 1 µM thapsigargin is capable of modulating inhibitory synaptic transmission, and the effects were ambivalent. Among 21 examined cells, eIPSCs decreased in 15 neurons and were augmented in 6 units; the kinetic parameters of these currents underwent no changes.