| 水稻线粒体DNA的提取与分析 |
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| 引用本文： | 涂君 朱英国. 水稻线粒体DNA的提取与分析[J]. 氨基酸和生物资源, 1997, 19(3): 4-7 |
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| 作者姓名： | 涂君 朱英国 |
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| 作者单位： | 武汉大学生命科学学院 |
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| 摘 要： | 为了研究水稻细胞质雄性不育的分子基础，我们比较了各种提取线粒体ＤＮＡ（ｍｔＤＮＡ）的方法，并提出了一些改进措施。以丛广４１Ａ、丛广４１Ｂ和杂种一代广优青为材料，对所提取的材料ｍｔＤ－ＮＡ进行了紫外扫描、ＯＤ值测定、电泳、酶切等分析，结果表明，以新鲜材料进行不连续蔗糖密度梯度超速离心对提取高纯度的线粒体ＤＮＡ效果较好。
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| 关 键 词： | 水稻 细胞质雄性不育 线粒体DNA |
The Extraction and Analysis of Mitochondrial DNA From Rice |
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| Tu Jun Zhu Yingguo. The Extraction and Analysis of Mitochondrial DNA From Rice[J]. Amino Acids & Biotic Resources, 1997, 19(3): 4-7 |
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| Authors: | Tu Jun Zhu Yingguo |
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| Abstract: | In order to study molecular mechanism of cytoplasmic male sterility(cms)of rice ( Oryza sativa L.), the authors have compared different methods for preparing mtDNAs from ChongGuan 41A, ChongGuan 41B and GuangYuQing rice. The resulted showed that discontinuous sucrose density gradient centrifugation method was effective for purifing mtDNA. Some physiochemical properties of rice mtDNAs were analysis. |
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| Keywords: | rice cytoplasmic male sterlility(cms) mitochondrial DNA(mtDNA) |
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