Identification of new minisatellites loci in Arabidopsis thaliana |
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| Authors: | Tourmente, S Lazreg, A Lafleuriel, J Tutois, S Cuvillier, C Espagnol, M Picard, G |
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| Affiliation: | UMR 6547 Biomove, Universite Blaise Pascal, 24 Avenue des Landais, F-63177 Aubiere Cedex, France; Corresponding author e-mail: tourmente@cicsum.univ-bpclemontfr |
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| Abstract: | In order to characterize new CG-rich minisatellites present in theArabidopsis thaliana genome, a genomic library wasscreened at low stringency with a probe containing nine repeated-units of aminisatellite (CMs1) previously identified. Both minisatellites andminisatellite-like elements were identified. The minisatellites, with atandemly-repeated structure, all contain the Arabidopsisthaliana-core sequence previously defined (Tourmenteet al., 1994). Both minisatellite andminisatellite-like sequences occur in the Arabidopsisgenome in low copy and are weakly polymorphic between ecotypes. The geneticmapping of these markers has shown that they are dispersed on the genome.YACs clones of the CIC library carrying these minisatellites andminisatellite-like sequences were identified.Key words:Arabidopsis thaliana, minisatellites, polymorphism |
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