Method for rapid removal of sodium dodecyl sulfate from polyacrylamide gels |
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| Authors: | H M Phillips |
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| Affiliation: | Department of Biochemistry, Neil Hellman Research Building, Albany Medical Cotteger Albany, New York 12208 USA |
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| Abstract: | A method is described for the isolation of hepatic microsomes by polyethylene glycol 6000 fractionation of the postmitochondrial fraction of liver homogenate. The procedure is simple and rapid requiring two centrifugation steps at 8000g for 10 min. The preparation has peptide patterns and levels of drug metabolic and other enzymatic activity similar to those of the microsomal fraction isolated by high-speed centrifugation and is referred to as polyethylene glycol 6000 microsomes. It is clarified with detergents and can serve as the starting material for the purification of microsomal proteins. |
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| Keywords: | Author to whom all correspondence should be sent. |
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