新型骨缺损斑马鱼模型构建及修复过程的OCT活体评价

动物模型广泛应用于人类疾病的病因、发病机制、防治技术和防治药物的探索研究中,新型人类疾病动物模型的开发将促进相关研究取得进展。本研究探索建立了一种基于斑马鱼的新型骨缺损模型,并利用光学相干层析成像(OCT)技术,对骨缺损模型的构建过程和修复过程进行活体评价。试验随机选取13条体型一致的成年斑马鱼作为研究对象,其中10条鱼用于构建骨缺损模型,剩余3条鱼作为对照。首先,对每一条正常斑马鱼的颅骨进行OCT扫描成像,然后利用自行研制的工具在模型组斑马鱼的视顶盖区域的颅骨上打开一个直径为200μm左右的孔,形成骨缺损。分别在第2、5、9、11、14、21天利用OCT对每一条斑马鱼的颅骨损伤情况进行评估。恢复21 d后的斑马鱼用于病理试验,将颅骨缺损区域的病理结果与OCT结果对照,新生骨清晰可见。试验结果表明,OCT可以高分辨率地活体评估骨缺损恢复的过程,OCT成像结果与病理切片结果高度匹配。综上所述,斑马鱼模型和OCT成像技术结合的策略能为骨缺损疾病的研究提供新的助力。

Construction of A New Bone Defect Zebrafish Model and in vivo Evaluation of the Repair Process by OCT

Animal models were widely used in the research of the etiology,pathogenesis,prevention,treatment of human diseases,and the development of new animal models of human diseases could greatly promote relevant research progress.In this study,a new bone defect model based on zebrafish was explored.Optical coherence tomography(OCT)was used to evaluate the building and repairing process of bone defect model in vivo.Thirteen adult zebrafish of the same size were used in this study,of which 10 were molded with bone defects and the remaining 3 were used as control.Firstly,each zebrafish skull was scanned by OCT,and then a hole about 200 microns in diameter was opened in the optic parietal region of the skull with a self-developed tool to form a bone defect.OCT was used to evaluate the skull injury of each zebrafish on the 2 nd,5 th,9 th,11 th,14 th and 21 st day,respectively.Twenty-one days after recovery,pathological experiments were performed to evaluate zebrafish skull.The pathological results of the defected skull were compared with OCT results.The experimental results showed that OCT could evaluate the process of bone defect recovery in vivo with high resolution.The new bone is clearly visible.The results of OCT imaging were highly matched with those of pathological results.In conclusion,the strategy of combining zebrafish model with OCT imaging technology will provide new impetus for the study of bone defect diseases.