Assessment of the microbial potential for nitrate-enhanced bioremediation of a JP-4 fuel-contaminated aquifer |
| |
| Authors: | J M Thomas C L Bruce V R Gordy K L Duston S R Hutchins J L Sinclair C H Ward |
| |
| Institution: | (1) Department of Environmental Science and Engineering, Rice University, Houston, Texas 77251, USA, US;(2) US Environmental Protection Agency, RS Kerr Research Laboratory, Ada, Oklahoma 74820, USA, US;(3) ManTech Environmental Technology, Ada, Oklahoma 74820, USA, US |
| |
| Abstract: | A site that was contaminated with JP-4 jet fuel was characterized microbiologically to assess the feasibility of nitrate-enhanced
bioremediation. The results of microcosm studies indicated that the mean pseudo zero-order rate constants for alkylbenzene
biodegradation and NO3
−-N removal were 1.2 and 2.4 mg L−1 per day, respectively. Several alkylbenzenes were removed to a greater extent in samples contaminated with residual JP-4
than in unexposed samples and samples downgradient of the spill; benzene was recalcitrant in all samples. Numbers of total
heterotrophs, JP-4-degraders, oligotrophs, total denitrifiers, denitrifiers growing in the presence of JP-4, estimates of
cell number by analysis of phospholipid fatty acids, direct counts and aerobic and anaerobic protozoa were determined; however,
numbers of microorganisms were not reliable predictors of alkylbenzene biodegradation activity. The presence of aerobic
and anaerobic protozoa suggests that protozoa may be active under a variety of different electron acceptor conditions. The
results of the characterization study indicated that the site was amenable to nitrate-enhanced bioremediation.
Received 12 March 1996/ Accepted in revised form 17 September 1996 |
| |
| Keywords: | : bioremediation site characterization nitrate alkylbenzenes aromatic hydrocarbons |
| 本文献已被 SpringerLink 等数据库收录！ |
|
