Identification on I-Ak molecules of a functional site recognized by proliferating T-lymphocytes |
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Authors: | Patrice C Dubreuil Daniel Z Birnbaum Danielle H Caillol Francois A Lemonnier |
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Institution: | (1) Centre d'Immunologie INSERM-CNRS de Marseille-Luminy, Case 906, 13288 Marseille Cedex 9, France |
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Abstract: | The inhibitory capacity of 17 monoclonal antibodies (m.Ab.) specific for the products of the I-A
k
subregion was evaluated in proliferative responses of B10.BR T-lymphocytes to GAT, Keyhole limpet hemocyanin, and ovalbumin. Considered in isolation, each m.Ab. mediated inhibitory effects of comparable magnitude on these three different proliferative responses. On the other hand, clear differences were observed when the magnitude of the inhibitory effects was compared from one m.Ab. to another. The m.Ab. were consequently classified as strong or moderate-to-weak inhibitors of T-cell proliferative responses. Evidence was simultaneously gained indicating the following: (a) the determinants recognized by different m.Ab. were expressed on the same molecules; (b) the differences in affinity of the m.Ab. for I-Ak positive cells did not explain their differences in inhibitory capacities; (c) conversely, the inhibitory capacity of each m.Ab. followed its ability to inhibit the cell surface fixation of Ia.17-specific 10-2.16 m.Ab.; (d) the strong inhibitory capacity of some m.Ab. was not related to a special ability to modulate cell surface Ia molecules. These results suggest that antigen recognition by T lymphocytes is preferentially restricted by a functional site of the I-Ak molecules related to the Ia.17 and Ia.1 specificities.Abbreviations EDTA
Ethylenediamine-tetraacetic acid disodium salt
- EHAA
Eagle's Hanks' amino acids medium
- FCS
fetal calf serum
- in polypeptide
G is glutamate, A, alanine, T, tyrosine
- HEPES
N-2-hydroxy-piperazine-N -2-ethane sulfonic acid
- kd
dissociation rate constant
- KLH
Keyhole limpet hemocyanin
- LPS
lipopolysaccharide
- m.Ab.
monoclonal antibodies
- NP-40
nonidet P-40
- PBS
phosphate buffered saline
- PBS-BSA
PBS supplemented with 1% bovine serum albumin
- PBS-BSA-NP-40
PBS-BSA supplemented with 0.5% NP-40
- RT
room temperature
- SEM
standard error of the mean
- s.c.
spleen cells |
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Keywords: | |
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