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Development of a serum-free and heat-sterilizable medium and continuous high-density cell culture
Authors:Yoshiki Minamoto   Koji Ogawa   Hideki Abe   Yoko Iochi  Koji Mitsugi
Abstract:We tried to establish a new serum-free and heat-sterilizable medium, based on our serum-free medium in which many lymphoblastoid cells and hybridoma could grow as well as in a conventional serum-containing medium.As is well-known, L-glutamine (L-Gln) is one of the most heat-labile but essential components for cell growth. As a substitute for L-Gln, dipeptide such as Gly-L-Gln or L-Ala-L-Gln, which was quite stable even after autoclaving, was found to be utilizable for mammalian cell growth. The L-Gln dipeptide-containing serum-free medium was quite stable in a solution even after storing at 37°C for 4 months. In the serum-free medium containing L-Ala-L-Gln, mouse hybridola could grow and produce more antibody than in RPMI 1640+10% FBS.
Keywords:glutamine peptide  high-density cell culture  human diploid fibroblast  hybridoma  lymphoblastoid cell line  serum-free
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