The cellulose-binding domain of endoglucanase A (CenA) from Cellulomonas fimi: evidence for the involvement of tryptophan residues in binding |
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| Authors: | Neena Din Ian J Forsythe Leslie D Burtnick Neil R Gilkes Robert C Miller Jr R Antony J Warren Douglas G Kilburn |
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| Institution: | Department of Microbiology, University of British Columbia, Vancouver, British Columbia, Canada V6T 1Z3.;Department of Chemistry, University of British Columbia, Vancouver, British Columbia, Canada V6T 1Z3. |
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| Abstract: | Cellulomonas fimi endo-β-1, 4-glucanase A (CenA) contains a discrete N-terminal cellulose-binding domain (CBDcenA)- Related CBDs occur In at least 16 bacterial glycanases and are characterized by four highly conserved Trp residues, two of which correspond to W14 and W68 of CBDcenA- The adsorption of CBDcenA to Crystalline cellulose was compared with that of two Trp mutants (W14A and W68A). The affinities of the mutant CBDs for cellulose were reduced by approximately 50- and 30-fold, respectively, relative to the wild type. Physical measurements indicated that the mutant CBDs fold normally. Fluorescence data indicated that W14 and W68 were exposed on the CBD, consistent with their participation in binding to cellobiosyl residues on the cellulose surface. |
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