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Identification of the mRNA coding for the ACTH-beta-lipotropin precursor in a human ectopic ACTH-producing tumor
Authors:T Tsukada  Y Nakai  H Jingami  H Imura  S Taii  S Nakanishi  S Numa
Affiliation:1. Second Division, Department of Internal Medicine Kyoto University Faculty of Medicine, Kyoto 606, Japan.;2. Department of Medical Chemistry, Kyoto University Faculty of Medicine, Kyoto 606, Japan.
Abstract:The mRNA coding for the ACTH-β-lipotropin precursor from a human ectopic ACTH-producing thymic carcinoid was identified by blot hybridization analysis with the bovine cDNA as a probe. The mRNA from the tumor had the same length (approximately 1,100 nucleotides) as that from the human pituitary. An additional hybridization-positive RNA species of a larger size was found in the tumor. Cell-free translation of the mRNA from the tumor as well as from the pituitary yielded a product with an apparent molecular weight of 38,000 that was reactive both with antibody to ACTH and with antibody to β-endorphin.
Keywords:β-LPH  β-lipotropin  γ-LPH  γ-lipotropin  γ-MSH  γ-melanotropin  IgG  immunoglobulin G  ACTH(1–24)  synthetic peptide with residues 1–24 of the amino acid sequence of corticotropin  SDS  sodium dodecylsulfate  DBM  diazobenzyloxymethyl
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