In vitro propagation of Glehnia littoralis from shoot-tips |
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Authors: | Noboru Hiraoka Miho Oyanagi |
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Institution: | (1) Niigata College of Pharmacy, 5-13-2 Kamishinei-cho, 950-21 Niigata, Japan |
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Abstract: | Shoot cultures of Glehnia littoralis F. Schmidt ex Miq. (Umbelliferae) were established by placing shoot tip explants on Linsmaier and Skoog medium with 1 M NAA and 10 M BAP. Shoots were multiplied on the basal medium supplemented with 0.3 M NAA and 3 M BAP and rooted on medium containing either 1 M IBA or 3–10 M IAA. Plantlets survived in pots without any covering. This unique characteristic of the plantlets was ascribed partly to a well-developed cuticle on the surface of the leaf and the small ratio of surface area to fresh weight of a leaf blade in comparison with those of other species whose plantlets needed coverings after potting. The regenerated plantlets were finally transferred to soil.Abbreviations IAA
potassium indole-3-acetate
- IBA
indole-3-butyric acid
- IPA
indole-3-propionic acid
- NAA
potassium 1-naphthaleneacetate
- 2,4-D
sodium 2,4-dichlorophenoxyacetate
- BAP
6-benzylaminopurine
- 2-iP
N6-(2-isopentenyl)adenine |
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