Sub-milliMorgan map of the proximal part of mouse chromosome 17 including the hybrid sterility 1 gene |
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| Authors: | S Gregorová M Mňuková-Fajdelová Z Trachtulec J ?apková M Loudová M Hoglund R Hamvas H Lehrach V Vincek J Klein J Forejt |
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| Institution: | (1) Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Vídeňská 1083, 142 20 Prague, Czech Republic, CS;(2) Department of Microbiology and Immunology, University of Miami School of Medicine, Miami, Florida 33101, USA, US;(3) Imperial Cancer Research Fund, London WC2A 3PX, UK, GB;(4) Abteilung Immunogenetik, Max-Planck-Institut für Biologie, D-72076 Tübingen, Germany, DE |
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| Abstract: | We have generated a high-resolution genetic map, 0.071 cM per backcross animal, of the 13 cM T–H2 region of the mouse Chromosome (Chr) 17. The map contains two phenotypic loci, T and Hst1, 12 RFLP markers, and 24 microsatellite loci. The Hst1 gene was mapped to a chromosomal interval contained within a single 580-kb YAC clone. The FFEH11 YAC is 0.44 cM long and
carries, besides the Hst1 gene, five polymorphic DNA markers and recombination breakpoints of six backcross animals. Two candidate genes for Hst1 were identified based on their location and testicular expression. These are Tbp and D17Ph4e. The sub-milliMorgan map of the T–H2 region revealed significant clustering of (CA)n loci. The clustering, if shown to be a common feature in the mouse genome, may cause gaps in the physical map of the mouse
genome.
Received: 11 September 1995 / Accepted: 9 October 1995 |
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