Evaluation of Chitosan/Alginate Beads Using Experimental Design: Formulation and <Emphasis Type="Italic">In Vitro</Emphasis> Characterization |
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Authors: | Sevgi Takka Aybige Gürel |
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Institution: | (1) Pharmaceutical Technology Department, Faculty of Pharmacy, University of Gazi, 06330, Etiler, Ankara, Turkey;(2) General Directorate Pharmaceuticals and Pharmacy, Ministry of Health of Turkey, 06060, Dışkapı, Ankara, Turkey |
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Abstract: | Bovine serum albumin-loaded beads were prepared by ionotropic gelation of alginate with calcium chloride and chitosan. The
effect of sodium alginate concentration and chitosan concentration on the particle size and loading efficacy was studied.
The diameter of the beads formed is dependent on the size of the needle used. The optimum condition for preparation alginate–chitosan
beads was alginate concentration of 3% and chitosan concentration of 0.25% at pH 5. The resulting bead formulation had a loading
efficacy of 98.5% and average size of 1,501 μm, and scanning electron microscopy images showed spherical and smooth particles.
Chitosan concentration significantly influenced particle size and encapsulation efficiency of chitosan–alginate beads (p < 0.05). Decreasing the alginate concentration resulted in an increased release of albumin in acidic media. The rapid dissolution
of chitosan–alginate matrices in the higher pH resulted in burst release of protein drug. |
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