| 48个烟草品种遗传多样性的RAPD分析 |
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| 引用本文： | 杨友才,余硕辉,尹晗琪. 48个烟草品种遗传多样性的RAPD分析[J]. 亚热带植物科学, 2008, 37(3): 10-14 |
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| 作者姓名： | 杨友才 余硕辉 尹晗琪 |
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| 作者单位： | 湖南农业大学 生物科学技术学院, 湖南 长沙 410128 |
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| 基金项目： | 湖南省自然科学基金，湖南农业大学校科研和教改项目 |
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| 摘 要： | 利用RAPD分子标记技术对48个烟草品种进行遗传多样性研究。从200个10bp的随机引物用RAPD方法筛选获得28个多态性引物,然后对48份烟草种质资源的基因组DNA进行扩增,共获得184条DNA扩增片断带。其中多态性带86条,平均多态检出率为46.7%。48份材料的遗传距离为0~7.81,采用UPGMA法聚类分析,可将其分为两大类群,即黄花烟草群与普通烟草群,后者又可分为4组。
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| 关 键 词： | 烟草 种质资源 遗传多样性 RAPD |
| 收稿时间： | 2008-04-10 |
Studies on Genetic Diversity of 48 Tobacco Varieties by RAPD Analysis |
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| YANG You-cai,YU Shuo-hui,YIN Hanqi. Studies on Genetic Diversity of 48 Tobacco Varieties by RAPD Analysis[J]. Subtropical Plant Science, 2008, 37(3): 10-14 |
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| Authors: | YANG You-cai YU Shuo-hui YIN Hanqi |
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| Affiliation: | College of Bioscience & Biotechnology, Hunan Agricultural University, Changsha 410128, Hunan China |
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| Abstract: | RAPD molecular-marker technique was used to detect the genetic diversity among 48 tobacco germplasms. The genomic DNA of 48 tobacco germplasms was tested with RAPD using 28 arbitrary 10-mer primers which were screened from 200 arbitrary 10-mer primers. The results showed that the 184 RAPD bands were obtained altogether with 86 bands of polymorphic ones. The percentage of polymorphic bands was 46.7%.The variation range of genetic distances was 0 to 7.81. These varieties were divided into N. tobacum group and N. rustica group by UPGMA cluster analysis based on RAPD molecular-markers data. N. tobacum group could be divided into 4 sub groups. |
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| Keywords: | RAPD |
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