Efficient and stable regeneration from protoplasts of <Emphasis Type="Italic">Cyclamen coum</Emphasis> Miller via somatic embryogenesis |
| |
Authors: | Anika Nadja Sabine Prange Margrethe Serek Melanie Bartsch Traud Winkelmann |
| |
Institution: | (1) Institute of Floriculture and Woody Plant Science, Gottfried Wilhelm Leibniz University of Hanover, Herrenhaeuser Str. 2, 30419 Hannover, Germany; |
| |
Abstract: | Embryogenic cultures of Cyclamen coum were established on solid media and in suspension, and their growth characteristics in response to different concentrations
of plant growth regulators (PGRs) were evaluated. Embryogenic cultures exhibited a high regeneration capacity of 876 somatic
embryos per gram fresh mass. Up to 4.24 × 105 protoplasts per gram of fresh mass were isolated from somatic embryos and embryogenic suspension cultures. Protoplasts derived
from both embryos and suspension cultures were successfully cultured in vitro and regenerated into plants via somatic embryogenesis.
Phenotypic analyses and flow cytometric measurements revealed that some regenerated plants were tetraploid. About 20% of the
protoplast-derived calluses used for regeneration were tetraploid, while tetraploidy was found in 0.9% of the plants regenerated
from the embryogenic cultures. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |