Improved culture conditions for somatic embryogenesis from Asparagus officinalis L. using an aseptic ventilative filter |
| |
Authors: | Takeo Saito Shuji Nishizawa Shigeo Nishimura |
| |
Institution: | (1) National Research Institute of Vegetables, Ornamental Plants and Tea, Ano, 514-23 Mie, Japan;(2) Nagano Prefecture Research Institute of Rural Industry, Suzaka, 328 Nagano, Japan |
| |
Abstract: | Summary Calli were induced from the crown of seedlings or lateral bud of young spears of Asparagus officinalis L. on Linsmaier and Skoog's (LS) solid-medium supplemented with 5 M 2,4-dichlorophenoxyacetic acid (2,4-D). Embryogenic callus was selected from induced calli and proliferated in LS liquid medium supplemented with 5 M 2,4-D. Non-vitrified somatic embryos were formed and efficiently developed into club-shaped embryos in LS hormone-free medium with 1 % gelrite in a culture vessel capped with an aseptic ventilative filter. Non-vitrified club-shaped embryos developed into normal plants when transferred to half-strength LS medium without hormones, and 0.8 % agar. Carbon dioxide concentration and moisture content inside the culture vessels were measured, and their effect on embryo development is discussed. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|