A DNA primer/probe system for the rapid and sensitive detection of Mycobacterium tuberculosis-complex pathogens |
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| Authors: | N T Ralphs S Garrett R Morse J B Cookson P W Andrew G J Boulnois |
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| Institution: | Department of Microbiology, University of Leicester, UK. |
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| Abstract: | A 1.5 kb EcoRI-BamHI restriction fragment from Mycobacterium tuberculosis was found to hybridize specifically with genomic DNA from M. tuberculosis-complex organisms. Primers were designed from the terminal sequences of this fragment and used to amplify uniquely M. tuberculosis-group DNA in a polymerase chain reaction. It is suggested that a combination of these primers and probe will prove a useful tool for the early diagnosis of tuberculous infections. |
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