Simultaneous extraction of proteins and DNA by an enzymatic treatment of the cell wall of <Emphasis Type="Italic">Palmaria palmata</Emphasis> (Rhodophyta) |
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Authors: | Yolaine Joubert Joël Fleurence |
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Institution: | (1) EMI EA 2663, Laboratoire Ecophysiologie Marine Intégrée, Faculté des Sciences et Techniques, Université de Nantes, 2 rue de la Houssinière, Nantes cedex 03, 44072, France |
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Abstract: | The extraction of proteins and DNA from seaweed tissue is difficult due to the presence of cell wall anionic polysaccharides
that, after cell disruption, remain in the extraction medium as hydrocolloidal compounds. These compounds increase medium
viscosity, thus limiting access to and, consequently, quantification of the soluble macromolecules such as proteins or DNA.
This study describes a protocol that enables the simultaneous isolation of proteins and DNA from the red seaweed Palmaria palmata. It is based upon a specific form of hydrolysis using a mixture of cellulase and xylanase at different concentrations. This
approach was carried out on samples collected during April and July, seasons in which differences in protein content have
previously been reported. Our results confirm this report and also show that protein yield depends on the enzyme concentration
used. As for DNA, this enzymatic digestion results in a much higher yield compared with the control. However, no seasonal
differences are found for DNA and there is no clear link between the increase in DNA yield and the enzyme mixture concentration. |
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Keywords: | Palmaria palmata Enzymatic digestion of cell wall Xylanase Cellulase Protein and phycoerythrin extraction DNA isolation |
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