Photon flux density and light quality induce changes in growth,stomatal development,photosynthesis and transpiration of <Emphasis Type="Italic">Withania Somnifera</Emphasis> (L.) Dunal. plantlets |
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Authors: | Sang-Ho Lee Rajesh Kumar Tewari Eun-Joo Hahn Kee-Yoeup Paek |
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Institution: | (1) Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University, Cheongju , 361-763, Republic of Korea |
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Abstract: | The aim of the study was to establish whether the quantity and the quality of light affect growth and development of Withania somnifera plantlets. We have studied growth and histo-physiological parameters stomatal characteristics, chloroplastic pigments concentrations,
photosynthesis, and transpiration (E)] of W. somnifera plantlets regenerated under various light intensities, or monochromatic light or under a mixture of two colors of light in
tissue culture conditions. Plantlets grown under a photon flux density (PFD) of 30 μmol m-2 s-1 showed greater growth and development than those raised under other PFDs. Chlorophylls and carotenoids, numbers of stomata,
rate of photosynthesis (PN) and transpiration (E), stomatal conductance (gs), and water use efficiency (WUE) increased with increasing the PFD up to 60 μmol m-2 s-1. Light quality also affected plantlets growth and physiology. Highest growth was observed under fluorescent and in a mixture
of blue and red light. Very few stomata were developed in any of the monochromatic light but under fluorescent or under a
mixture of two colors stomatal numbers increased. Similarly, gs, E, PN, and WUE were also higher under fluorescent light and under a mixture of red and blue light. Regressional analysis showed
a linear relationship between PN (r
2 = 70) and gs and between E (r
2 = 0.95) and gs. In conclusion, both the quality and the quantity of light affect growth of plantlets, development of stomata and physiological
responses differently depending on the intensity and the wavelength of light. |
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Keywords: | Ashwagandha Indian ginseng In-vitro culture Photosynthesis Spectral intensity Spectral quality Stomatal development Transpiration Water use efficiency |
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