ITS sequences and speciation on far easternIndigofera (Leguminosae) |
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Authors: | Byoung-Hee Choi Joo-Hwan Kim |
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Institution: | (1) Department of Biology, Inha University, Yonghyeon-dong, Nam-ku, 402-751 Incheon, Korea;(2) Present address: Department of Biology, Taejon University, 300-716 Taejon, Korea |
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Abstract: | The internal transcribed spacer (ITS) regions of 18–26S nuclear ribosomal DNA was sequenced to address phylogenetic relationships
and to measure the extent of differentiation among six species of the Far EasternIndigofera. ITS 1 had 230–240 base pairs (bp) long while ITS 2 had 211–213 bp long. The 5.8S rRNA coding region was 161 bp long. Sequence
divergence calculated by Kimura's two parameter method between species ranged from 0.00 to 13.49%. A single most parsimonious
tree was produced from 77 variable nucleotide sites, which had a consistency index of 0.97 and a retention index of 0.83.
ITS sequence data suggested that the continental species ofI. kirilowii (2n=16) is diverged from the common ancestor of other species at first, and then the island species ofI. decora (2n=48) andI. venulosa and the Korean Peninsular species ofI. grandiflora (2n=16) andI. koreana (2n=32) are diverged from the ancestor. The molecular data supports that the speciation in the Far EasternIndigofera occurred with polyploidization from continental ancestor to peripheral peninsular and island species. |
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Keywords: | Far East Indigofera Internal transcribed spacer sequences Leguminosae Nuclear ribosomal DNA Speciation |
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