| 黄孢原毛平革菌基因启动子的分离与鉴定 |
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| 引用本文： | 李维,张义正.黄孢原毛平革菌基因启动子的分离与鉴定[J].生物工程学报,2000,16(5):599-602. |
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| 作者姓名： | 李维 张义正 |
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| 作者单位： | 1. 四川师范大学生物系
2. 四川大学生命科学学院,成都,610064 |
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| 基金项目： | 国家自然科学基金资助项目(39430020)。 |
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| 摘 要： | 利用启动子探针型载体pSUPV8直接在大肠杆菌(Escherichia coli)中分离黄孢原毛平革菌(Phanerochaete chrysosporium)基因启动子片段，获得6个潮霉素抗性(Hyg-r)重组子。对重组子CH2、CH6进行序列分析，结果发现它们都存在真核生物基因启动子的保守序列；用原生质体转化法将其转化黄孢原毛平革菌，仅pCH6获得了潮霉素抗性转化子；PCR和斑点杂交分析表明，pCH6已成功导入黄孢原毛平革菌，并启动潮霉素抗性基因的表达。
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| 关 键 词： | 大肠杆菌，黄孢原毛平革菌，基因启动子，序列分析，启动子探针型载体 |
| 文章编号： | 1000-3061-(2000)05-0599-04 |
| 修稿时间： | 1999-11-02 |
Isolation and Characterization of Promoters from Phanerochaete chrysosporium |
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| LI Wei,ZHANG Yi-Zheng.Isolation and Characterization of Promoters from Phanerochaete chrysosporium[J].Chinese Journal of Biotechnology,2000,16(5):599-602. |
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| Authors: | LI Wei ZHANG Yi-Zheng |
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| Institution: | LI Wei
,ZHANG Yi-Zheng
;(Colgege of Life Science, Sichuan University, Chengdu 610064) |
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| Abstract: | Promoter-probe vector pSUPV8 was used to clone promoters from Phanerochaete chrysosporium directly in Escherichia coli. Six hygromycin B-resistant recombinants were obtained and two inserted fragments of them were sequenced. The results indicated that they contain several sequences similar to eukaryotic cis regulatory elements. Only pCH6 could transform P, chrysosporium into hygromecin B resistance. PCR and dot hybridization analysis indicated that it was introduced into P. chrysosporium successfully and leaded to the HmB-resistance. The results demonstrated that HmB-resistant gene (hph) could be used as an ideal reporter gene for P. chrysosporium transformation. |
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| Keywords: | E coli P chrysosporium gene promoter promoter-probe vector sequencing |
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