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| 多色荧光原位杂交对昆明山海棠和丙烯酰胺诱发微核染色体组成的研究 | |
| 引用本文： | 杨明杰,曹佳. 多色荧光原位杂交对昆明山海棠和丙烯酰胺诱发微核染色体组成的研究[J]. 细胞生物学杂志, 2000, 22(4): 212-215 |
| 作者姓名： | 杨明杰 曹佳 |
| 作者单位： | 第三军医大学预防医学系分子毒理实验室,重庆 |
| 基金项目： | 本课题由国家自然科学基金资助(基金编号39400114)。Project supported by National Natural Science Foundation of China(No.39400114). |
| 摘 要： | 采用着丝粒和端粒DNA探针多色荧光原位杂交，分析了昆明山海棠和丙烯酰胺诱导的NIH3T3细胞微核的染色体组成。结果表明，昆明山海棠和丙烯酰胺诱导的由整条染色体组成的微核分别可达70.7％和65.9％，提示昆明山海棠和丙烯酰胺均有较强的非整倍体毒性。 |
| 关 键 词： | 多色荧光原位杂交 昆明山海棠 微核染色体 |
| 修稿时间： | 1999-01-28 |
STUDY ON CHROMOSOMAL COMPOSITION OF MICRONUCLEI INDUCED BY TRIPTER YGIUM HYPOGLA UCUM (LEVEL)HUTCH AND ACRYLAMIDE WITH MULTICOLOR FLUORESCENCE IN SITU HYBRIDIZATION |
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| YANG Mng Jie CAOJia. STUDY ON CHROMOSOMAL COMPOSITION OF MICRONUCLEI INDUCED BY TRIPTER YGIUM HYPOGLA UCUM (LEVEL)HUTCH AND ACRYLAMIDE WITH MULTICOLOR FLUORESCENCE IN SITU HYBRIDIZATION[J]. Chinese Journal of Cell Biology, 2000, 22(4): 212-215 | |
| Authors: | YANG Mng Jie CAOJia |
| Abstract: | Multicolor fluorescence in situ hybridization using simultaneously a combination of DNA probes for the centromerically mouse minor satellite DNA and the telomeric hexamer repeat (TTAGGG)was applied to analyze the composition of micronu-clei(MN)in mouse NIH 3T3 fibroblasts induced by tripterygium hypoglaucum(level)hutch(THH)and acrylamide(AA). The results showed that 70.7% of THH-induced MN and 65.9% of AA-induced MN contained whole chromosomes,suggesting both THH and AA have aneugenic poison. Multicolor FISH with centrctneric and telomeric DNA probes shows as an accurate technique for analysis of the chromoscmal composition of MN. |
| Keywords: | Multicolor fluorescence in situ hybridization Micronuclei Tripterygium hypoglaucum(Level) Hutch Acrylamide Aneugen |
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