Loss of viability correlates with altered acid phosphatase activity in suspended strawberry cell fractions after excessive agitation

Acid phosphatase (EC 3.1.3.2.) activity in all fractions and growth phases of strawberry cell culture was 3-fold higher in the excessively agitated cells compared to a control. The stressed cells lost 9% of viability in the lag phase. Concomitantly the enzyme activity ratio of the lysosome fraction to the crude organelle mixture decreased (-33%) whereas the activity ratio of the cytosol fraction to the crude organelle mixture increased (+30%). This change in the enzyme activity of the ratio of the fractions was not observed in the exponential and stationary phase where the loss of viability was only 4 to 5%.