Interactions of the Mont Terri Opalinus Clay Isolate Sporomusa sp. MT-2.99 with Curium(III) and Europium(III) |
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| Authors: | Henry Moll Laura Lütke Velina Bachvarova Andrea Cherkouk Sonja Selenska-Pobell Gert Bernhard |
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| Affiliation: | 1. Institute of Resource Ecology, Helmholtz-Zentrum Dresden-Rossendorf e.V., Dresden, Germanyh.moll@hzdr.de;3. Institut für Radio?kologie und Strahlenschutz (IRS), Leibniz Universit?t Hannover, Hannover, Germany;4. Department of Developmental Biology/Center for Medical Biotechnology, University of Duisburg-Essen, Essen, Germany;5. Institute of Resource Ecology, Helmholtz-Zentrum Dresden-Rossendorf e.V., Dresden, Germany |
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| Abstract: | Bacterial cell walls have great potential to influence the speciation and mobility of actinides and lanthanides in the environment. In this study we explored the unknown interaction between Cm(III)/Eu(III) and cell-suspensions of Sporomusa sp. MT-2.99, a novel isolate recovered from Opalinus Clay (Mont Terri, Switzerland). The Cm(III)/Eu(III) binding by the cell surface functional groups was studied by potentiometry combined with time-resolved laser-induced fluorescence spectroscopy (TRLFS). This article provides stability constants of Cm(III)/Eu(III) complexed by cell surface functional groups. We could show that as a function of pH Cm(III)/Eu(III) binding occurred to hydrogen phosphoryl, carboxyl and deprotonated phosphoryl sites. Both metals showed a similar interaction process consisting of surface complexation (major) with high thermodynamic stability and an irreversible binding within the cell envelope (minor). |
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| Keywords: | bacteria complexation curium europium Sporomusa sp. TRLFS potentiometry |
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