Epiderstatin and Its Related Glutarimide Antibiotics Inhibit the Cell Growth Induced by Mitogen Stimulation |
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| Authors: | Tadamichi Sonoda Hiroyuki Osada Junji Magae Kiyoshi Isono |
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| Institution: | 1. Antibiotics Laboratory, RIKEN, The Institute of Physical and Chemical Research, Wako, Saitama 351-01, Japan;2. Department of Bio engineering, Tokyo Institute of Technology, Meguro-ku, Tokyo 152, Japan |
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| Abstract: | To investigate the immunosuppressive effects of glutarimide antibiotics including a new antibiotic named epiderstatin, we tested these antibiotics for inhibition of the blastogenesis of mouse spleen cells induced by mitogen stimulation (concanavalin A or lipopolysaccharide). The inhibitory activity was measured by colorimetric MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay. Among the glutarimide antibiotics tested, epiderstatin and acetoxycycloheximide especially strongly inhibited the blastogenesis of mouse spleen cells induced by concanavalin A and lipopolysaccharide, however, selectivity between T and B lymphocytes was not observed. |
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| Keywords: | microsomes (?)-menthone kinetic analysis |
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