Intracellular Distribution and Some Properties of β-Glucosidases of a Cellulolytic Pseudomonad |
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| Authors: | Jun-Ton Hwang Hiroshi Suzuki |
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| Institution: | Department of Botany, Faculty of Science, Tokyo Kyoiku University, Otsuka, Tokyo |
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| Abstract: | Two distinct forms of β-glucosidase, A and B, were found to occur in the cells of Pseudomonas fluorescens var. cellulosa : A was membrane-bound, while B cytosolic. They differed also from each other in some properties, such as molecular size, kinetic parameters, and susceptibility to various compounds. β-Glucosidase B was partially purified and studied especially of its substrate specificity. The results indicated that it may be an atypical β-glucosidase which possesses a certain character of exo-cellulase. |
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| Keywords: | short-chain inulin-like fructans short-chain fatty acids lactobacilli trinitrobenzene sulfonic acid (TNBS) colitis rats |
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