Structure and Hypotensive Effect of Flavonoid Glycosides in Kinkan (Fortunella japonica) Peelings |
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| Authors: | Hiroyasu Kumamoto Yoshiharu Matsubara Yoshitomi Iizuka Kozo Okamoto Katsumi Yokoi |
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| Institution: | 1. Department of Applied Chemistry, Faculty of Science and Engineering, and, Research Institute of Food Science, Kinki University, Kowakae, Higashiosaka, 577, Japan;2. Kinki University School of Medicine, Sayamacho, Minamikawachi, 588, Japan;3. Japan Sangaria Beverage Co., Ltd., Nakano, Higashisumiyoshi-ku, Osaka, 546, Japan |
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| Abstract: | An attempt was made to isolate the hypotensive substances from a hot water extract of kinkan. Eight flavonoid glycosides were isolated by repeated chromatography and by gel filtration after extracting with n-butanol and treating with lead subacetate. Their structures were established to be 6,8-di-C-glucosylapigenin (1), 3,6-di-C-glucosylacacetin (2), 2″-O-α-l-rhamnosyl-4′-O-methyl-vitexin (3), 2″-O-α-l-rhamnosyl-4′-O-methylisovitexin (4), 2″-O-α-l-rhamnosylvitexin (5), 2″-O-α-l-rhamnosylorientin (6), 2″-O-α-l-rhamnosyl-4′-O-methylorientin (7) and ponicilin (8) by UV. MS, 1-NMR and 13C-NMR spectroscopy, and by sugar analysis. Each component was intravenously injected in SHR-SP (0.5 ~ 1.0 mg/100 g of body weight), 1, 2, 5 and 6 were found to lower the rat blood pressure.Among these compounds, 2, 3, 4, 6 and 7 were new flavone glycosides. |
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