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Isolation and Characterization of Globulin from Seeds of Amaranthus hypochondriacus L.
Authors:Yotaro Konishi  Yuko Fumita  Kayoko Ikeda  Kazutoshi Okuno  Hidetsugu Fuwa
Institution:1. Department of Food and Nutrition, Faculty of Science of Living, Osaka City University, 3-3-138, Sugimoto, Sumiyoshi-ku, Osaka 558, Japan;2. Field Crop Division, Hokuriku National Agricultural Experiment Station, Joetsu, Niigata 943-01, Japan
Abstract:Seeds of grain amaranths contain a high amount (about 60% of total nitrogen) of albumin and globulin and a trace amount of prolamin. From salt-soluble extracts of A. hypochondriacus seeds, a globulin (440,000 in apparent molecular weight and  /></span>) was purified by Sepharose 6B gel and DEAE-cellulose column chromatographies. The protein comprised at least four kinds of subunits whose molecular weights were 36,000, 32,000, 20,000 and 18,000, respectively. The amino acid composition of the globulin was almost similar to those of soybean and oat globulins.</td>
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