Genetic Mapping of Cold Resistance Gene of Escherichia coli |
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Authors: | Shinichi Kawamoto Shinji Tokuyama Kenji Aoyama Shigetaka Yashima Yoshitomo Eguchi |
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Affiliation: | Department of Microbial Engineering and Technology, Faculty of Agriculture, Hokkaido University, Sapporo 060, Japan |
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Abstract: | Using cold resistant mutants, MET1 and MET2, obtained from Escherichia coli K-12, genetic mapping of the cold resistance gene(s) of E. coli was performed by the conjugation and transduction techniques. The gene(s) was confirmed to be located close to trpB at 28 min (revised chromosome linkage map, 1983) on the E. coli chromosome. |
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