Thioredoxin-catalyzed Refolding of Recombinant Protein: Refolding of Human Pro-urokinase |
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| Authors: | Katsuzumi Okumura Hideto Wakayama Yoshiaki Miyake Keiichi Murayama Toshio Miyake Koji Seto |
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| Affiliation: | 1. Laboratory of Biological Chemistry, Faculty of Bioresources, Mie University, Tsu, Mie 514, Japan;2. Biotechnology Research Laboratory, Tosoh Co., Ltd., Ayase, Kanagawa 252, Japan |
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| Abstract: | We have isolated four independent genomic DNA fragments encoding a rice storage protein, glutelin, by using its cDNA as a probe. Restriction mapping and sequencing analyses showed that all four clones encode type II mRNA of glutelin and have very similar nucleotide sequences. However, several differences were found among the four clones with respect to the nucleotide sequences of their coding and 5′-upstream regions, some of which confirmed corresponding changes in the amino acid sequences of the encoded proteins. These results indicate that the genes encoding type II mRNA species of glutelin constitute a multi-gene family of closely related members. |
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