Nucleotidylation of Aminoglycoside Antibiotics by Bacillus brevis |
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| Authors: | Hideo Shirafuji Makoto Kida Tsunehiko Asako Masahiko Yoneda |
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| Institution: | Central Research Division, Takeda Chemical Industries, Ltd., Yodogawa-ku, Osaka 532, Japan |
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| Abstract: | Biochemical transformations of aminoglycoside antibiotics by Bacillus species were examined. Among 39 strains of 8 Bacillus species, 4 strains of B. brevis were found to inactivate several aminoglycoside antibiotics: neamine, xylostasin, butirosin A and kanamycin A.In the presence of Mg+2 and ATP, the cell-free extracts of B. brevis IFO 12334 catalyzed the transformation of xylostasin to its inactive form. The structure of this inactivated xylostasin was determined to be 4′-O-monoadenylylxylostain from the 13C-NMR spectra, and from biochemical and spectroscopic studies. |
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