Effect of Emodin on the Mutagenicity of 3-Amino-1-methyl-5H-pyrido[4,3-b]indol toward Salmonella |
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| Authors: | Atsuko Kushi Akira Koiwai Daisuke Yoshida Fujio Gotō |
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| Institution: | 1. Centrai Research Institute, The Japan Tobacco &2. Salt Public Corporation, 6-2 Umegaoka, Midori-ku, Yokohama, Kanagawa 227, Japan |
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| Abstract: | Mass spectra of N-trifluoroacetyl n-butyl ester (BTFA) of ornithinoalanine (OAL) and lanthionine (LAN) were compared with those of the BTFA derivatives of lysinoalanine (LAL) and the related amino acids. A difference of m/z 14, corresponding to one methylene group, was found in each pair of characteristic fragments between BTFA-OAL and BTFA-LAL. A temperature-programmed gas-liquid chromatography and gas chromatography-mass spectrometry were developed to analyze BTFA-LAN, BTFA-OAL and BTFA-LAL. More LAL and LAN were formed in α-lactalbumin than lysozyme by high-temperature treatment in water. OAL was detected in lysozyme treated at 100° and 120°C in alkali solution, but not in α-lactalbumin. |
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