Isolation and Chemical Characterization of a Mating Pheromone Produced by Saccharomyces kluyveri |
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| Authors: | Akira Sakurai Yukiharu Sato Keun Hyung Park Nobutaka Takahashi Naohiko Yanagishima Isao Banno |
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| Affiliation: | 1. The Institute of Physical and Chemical Research, Wako-shi, Saitama;2. Department of Biology, Nagoya University, Chikusa-ku, Nagoya;3. Institute for Fermentation, Osaka, Yodogawa-ku, Osaka |
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| Abstract: | The pullulanase gene (pul) of Klebsiella aerogenes was transferred in vivo to Escherichia coli by using RP4:: Mu cts. The pul gene was expressed in E. coli, although the level of pullulanase activity in E. coli was lower than that in K. aerogenes, and the Pul+ transconjugants were relatively unstable in an unselective medium. Production of pullulanase, which is used to make maltose from starch, was induced in E. coli by pullulan, waxy maize amylopectin, soluble starch and maltose. When the transconjugant cells of E. coli were grown with pullulan or maltose, most pullulanase was produced intracellularly, whereas K. aerogenes produced pullulanase extracellularly. Retransfer of the pulk gene from E. coli to K. aerogenes by conjugation resulted in an increase of the production of extracellular pullulanase. |
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