Detection of Polygalacturonase in Kiwifruit during Ripening |
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| Authors: | Isao Soda Tadao Hasegawa Takao Suzuki Nagao Ogura |
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| Institution: | 1. Department of Agricultural Chemistry, Tokyo University of Agriculture, Setagaya-ku, Tokyo 156 Japan;2. Department of Agricultural Chemistry, Faculty of Horticulture, Chiba University, Matsudo, Chiba 271 Japan |
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| Abstract: | Oligosaccharides produced during the course of the hydrolysis of 25% N-acetylated chitosan by Streptomyces griseus chitinase were fractionated by CM-Sephadex C-25 and Toyopearl HW-40F column chromatographies. Sugar compositions and sequences of main oligosaccharides were identified by N-acetylation, exo-splitting with β-GlcNAcase and β-GlcNase, and nitrous acid degradation. In addition to N-acetylated saccharides, GlcNAc, (GlcNAc)2, and (GlcNAc)3, hetero-chitooligosaccharides such as GlcN · GlcNAc, GlcN · GlcNAc · GlcNAc, GlcN · GlcN · GlcNAc, GlcN · GlcNAc · GlcNAc · GlcNAc, GlcNAc · GlcN · GlcNAc · GlcNAc, GlcN · GlcNAc · GlcN · GlcNAc, and GlcN · GlcN · GlcNAc · GlcNAc were identified. These results indicate that Streptomyces griseus chitinase specifically cleaves the N-acetyl-β-d-glucosaminidic linkages in partially N-acetylated chitosan. |
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