Mutagenesis of soybean (Glycine max (L.) Merr.) and the isolation of non-nodulating mutants |
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| Affiliation: | 1. Department of Pharmacology of Changzhi Medical College, Changzhi City, Shanxi Province 046000, People''s Republic of China;2. Department of Dermatology of Heping Hospital Affiliated to Changzhi Medical College, Changzhi City, Shanxi Province 046000, People''s Republic of China;3. Oncology Department of Changzhi People''s Hospital, Changzhi City, Shanxi Province 046000, People''s Republic of China;4. Urinary Surgery of Heping Hospital Affiliated to Changzhi Medical College, Changzhi City, Shanxi Province 046000, People''s Republic of China;5. Molecular Design and Nanotechnology Laboratory of Institute of Biomedical Engineering, Chinese Academy of Medical Sciences, 236 Baidi Road, Nankai District, Tianjin City 300192, People’s Republic of China |
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| Abstract: | Soybean seeds (cv. Bragg) were mutagenized with either ethyl methanesulphonate (EMS), gamma-rays or sodium azide (NaN3). EMS was the most efficient in generating chlorophyll-deficient variants in the M2. NaN3 was not an effective mutagen. Approximately 25 200 M2 plants were screened for absence of nodulation after inoculation with Bradyrhizobium japonicum strain CB1809 (=USDA136). Three stable non-nodulation mutants were recovered and designated nod49, nod772 and nod139. Mutant nod49 was isolated from a mixture of M2 populations and this mutant also failed to nodulate upon inoculation with Rhizobium fredii strain USDA257, which nodulated the parent cultivar. Mutants nod772 and nod139 came from segregating EMS-derived M2 families, indicating that these mutations were a result of mutagenesis. Inheritance of the non-nodulation character has been demonstrated through to the M5, M4 and M3 generations for nod49, nod772 and nod139, respectively. |
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