Electron spin resonance detection of oxygen-centred radicals in murine macrophages stimulated with bacterial endotoxin |
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| Authors: | S K Jackson J M Stark C C Rowlands J C Evans |
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| Institution: | Department of Medical Microbiology, University of Wales College of Medicine, Health Park, Cardiff, U.K. |
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| Abstract: | The production of oxygen radicals by Bacille-Calmette-Guerin primed mouse macrophages stimulated with bacterial endotoxin has been investigated. Superoxide radicals were spin-trapped in this system with dimethylpyrroline-N-oxide after a lag period of 20-40 minutes. The electron spin resonance signals due to the superoxide radical adduct could be inhibited by superoxide dismutase but not by catalase. |
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