Morphological characterization of a human glioma cell l ine |
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Authors: | Camila?ML?Machado André?Schenka José?Vassallo Wirla?MSC?Tamashiro Estela?M?Gon?alves Selma?C?Genari Email author" target="_blank">Liana?VerinaudEmail author |
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Institution: | 1.Department of Microbiology and Immunology, Biology Institute,State University of Campinas,Campinas,Brazil;2.Department of Pathology, School of Medicine,State University of Campinas,Campinas,Brazil;3.Department of Cellular Biology, Biology Institute,State University of Campinas,Campinas,Brazil |
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Abstract: | A human malignant continuous cell line, named NG97, was recently established in our laboratory. This cell line has been serially
subcultured over 100 times in standard culture media presenting no sign of cell senescence. The NG97 cell line has a doubling
time of about 24 h. Immunocytochemical analysis of glial markers demonstrated that cells are positive for glial fibrillary
acidic protein (GFAP) and S-100 protein, and negative for vimentin. Under phase-contrast microscope, cultures of NG97 showed
cells with variable morphological features, such as small rounded cells, fusiform cells (fibroblastic-like cells), and dendritic-like
cells. However, at confluence just small rounded and fusiform cells can be observed. At scanning electron microscopy (SEM)
small rounded cells showed heterogeneous microextentions, including blebs and filopodia. Dendritic-like cells were flat and
presented extensive prolongations, making several contacts with small rounded cells, while fusiform cells presented their
surfaces dominated by microvilli. |
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