Acetyl-coenzyme A: arylamine N-acetyltransferases in microorganisms: screening and isolation of an enzyme from Bacillus cereus |
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Authors: | M J Hasmann P H Seidl G Engelhardt K H Schleifer |
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Institution: | (1) Lenrstuhl für Mikrobiologie, Technische Universität München, Arcisstr. 21, D-8000 München 2, Federal Republic of Germany;(2) Bayerische Landesanstalt für Ernährung, Menzingerstr. 54, D-8000 München 19, Federal Republic of Germany |
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Abstract: | The raw extracts of a series of microorganisms were screened for the presence of acetyl-coenzyme A: arylamine N-acetyltransferase (AAAT) using a radioactive assay with 3H-acetyl-coenzyme A and aniline as substrates. Enzyme activities were primarily detected in the soluble fractions of Bacillus and Nocardia species, and in some further soil organisms. Only strains of Bacillus cereus were able to acetylate 4-nitroaniline and 3,5-dimethyl-4-nitroaniline. The fermentation conditions for the production of the enzyme were optimized. The AAAT from one strain of Bacillus cereus was purified 24-fold and characterized.Abbreviations AAAT
acetyl-coenzyme A: arylamine N-acetyltransferase
- AcP
acetylphosphate
- CoA
coenzyme A
- EDTA
ethylenediaminetetra-acetic acid
- PTA
phosphotransacetylase |
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Keywords: | Arylamine N-acetyltransferase Metabolism of aromatic amines Soil microorganisms Bacillus cereus |
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