Application of an immunoaffinity-based preconcentration method for mass spectrometric analysis of the O-chain polysaccharide of Aeromonas salmonicida from in vitro- and in vivo-grown cells |
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| Authors: | Zhan Wang Xin Liu Elizabeth Garduño Rafael A Garduño Jianjun Li & Eleonora Altman |
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| Institution: | Institute for Biological Sciences, National Research Council of Canada, Ottawa, ON, Canada;;Institute for Marine Biosciences, National Research Council of Canada, Halifax, NS, Canada;and;Dalhousie University, Halifax, NS, Canada |
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| Abstract: | In this study, application of magnetic beads (Dynabeads) coated with Aeromonas salmonicida lipopolysaccharide-specific polyclonal antisera to MS-based characterization of bacterial lipopolysaccharides has been evaluated. The results showed that the affinity-based preconcentration strategy resulted in at least a 100-fold increase in the detection of sensitivity, affording direct capillary electrophoresis (CE)-MS analysis of A. salmonicida lipopolysaccharide O-chain polysaccharide from in vitro- cultured cells. Subsequent CE-MS analysis of in vivo -grown cells of A. salmonicida confirmed significant changes in the structure of the lipopolysaccharide O-chain polysaccharide as a result of in vivo cultivation. |
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| Keywords: | Aeromonas salmonicida MS lipopolysaccharide identification |
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