Mechanism of regulating the expression of λN gene by ribosomal protein at translational level |
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Authors: | LI Muyang WENG Manli TONG Kezhong |
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Institution: | (1) Institute or Genetics, Chinese Academy of Sciences, 100101 Beijing, China |
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Abstract: | In ribosomal protein S12 mutant or L24 mutant the expression of λN gene was depressed at translational level. To study its
mechanism the λN gene region of λN -lacZ gene fusion was trimmed from its 5′ end to 3′ end with DNA exonuclease III (DNA cxoIII) in order to alter the TIR (translational
initiation region) and the ding region of λN gene. After DNA sequencing 23 species of different λN-lacZ fused genes were obtained.
The β-galactosidase activities of these deletants in ribosomal protein mutant were compared with that in wild type strain.
The result indicated that (i) S12 mutant could affect 305 subunit’s binding to the TIR of λN gene messenger and cause the
difficulty in forming 30s initiation complex and then decrease the efficiency of translational initiation; (ii) in S12 mutant
the coding region of λN gene alw affected the expression λN gene; (iii) in L24 mutant the inhibition of λN gene expression
was not related to translational initiation and the 5′ end of the coding region of λN gene, but related to the 3′ end of λN
gene.
Project supported by the National Natural Science Foundation of China (Grant Nos. 39480014, 39570162) and Chinese Academy
of Sciences. |
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Keywords: | ribosomal protein mutant translational control λ N gene |
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