Energy metabolism of Heliobacterium modesticaldum during phototrophic and chemotrophic growth |
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Authors: | Kuo-Hsiang Tang Hai Yue Robert E Blankenship |
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Affiliation: | (1) Department of Biology, Washington University in St. Louis, One Brookings Drive, Campus Box 1137, 63130 St. Louis, Missouri, USA;(2) Department of Chemistry, Washington University in St. Louis, One Brookings Drive, Campus Box 1137, 63130 St. Louis, Missouri, USA |
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Abstract: | Background Heliobacterium modesticaldum is a gram-positive nitrogen-fixing phototrophic bacterium that can grow either photoheterotrophically or chemotrophically but not photoautotrophically. Surprisingly, this organism is lacking only one gene for the complete reverse tricarboxylic acid (rTCA) cycle required for autotrophic carbon fixation. Along with the genomic information reported recently, we use multiple experimental approaches in this report to address questions regarding energy metabolic pathways in darkness, CO2 fixation, sugar assimilation and acetate metabolism. |
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