Role of the kallikrein-kinin system in glandular secretion

Microsomes were prepared from liver, lung and kidney of rats and rabbits using a Ca⁺² aggregation method. Microsomal protein yield from the lung of both species was higher by this method of preparation as compared with ultracentrifuged samples. Specific activities of rat and rabbit pulmonary p-chloro-N-methylaniline (CMA) demethylase, biphenyl 4-hydroxylase and rat pulmonary TPNH cytochrome c reductase also were decreased. Specific activities of rabbit hepatic TPNH cytochrome c reductase, CMA N-demethylase, UDP-glucuronyltransferase and biphenyl hydroxylase were decreased by calcium aggregation Renal enzyme activities were unchanged by this method of preparation. These data indicate an apparent species and organ difference in microsomal enzyme response to calcium aggregation.