Genetics of alcohol dehydrogenase in Saccharomyces cerevisiae: I. Isolation and genetic analysis of adh mutants |
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Authors: | M Ciriacy |
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Institution: | Forstbotanisches Institut der Universität Freiburg i.Br., 78 Freiburg i.Br., W. Germany |
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Abstract: | On the basis of allyalcohol resistance, Saccharomyces cerevisiae mutanta were isolated that were deficient in alcohol dehydrogenase (ADH). The mutants were divided into three classes by their different ADH isozyme pattern obtained after starch-gel electrophoresis: adc mutants that did not produce the constitutive ADH, adr mutants from which the glucose repressible enzyme (ADHII) was absent, and adm mutants deficient in ADH activity associated with the mitochondria.Genetic analysis showed that two genes control synthesis of the glucose repressible enzyme ADHII, one gene the constitutive ADHI and a fourth nuclear gene the mitochondrial ADH. None of these four genes showed any linkage.The various mutant types did not show drastic effects on yeast growth on media containing glucose or ethanol as sole carbon sources. |
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Keywords: | ADH alcohol dehydrogenase NPD non-parental ditype PD parental ditype RD respiration-deficient T tetratype YEP yeast extract plus bactopeptone |
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