Purification and properties of glycollate oxidase from Pisum sativum leaves |
| |
| Authors: | Michael W. Kerr David Groves |
| |
| Affiliation: | Shell Research Limited, Woodstock Laboratory, Sittingbourne, Kent ME9 8AG USA |
| |
| Abstract: | A rapid and efficient method for the isolation of glycollate oxidase from pea leaves is described. The method utilizes the unusually high isoelectric point (pH 9·6) which has been determined for the enzyme using isoelectric focusing. The enzyme is apparently homogeneous by polyacrylamide gel electrophoresis and has a MW of ca 100000. Some properties of the enzyme are described. |
| |
| Keywords: | Leguminosae pea glycollate oxidase purification properties isoelectric focusing MW |
| 本文献已被 ScienceDirect 等数据库收录！ |
