| Abstract: | α-Amylase, limit dextrinase and α-glucosidase were induced by gibberellic acid in barley grain from which the embryos had been excised. The responses to different concentrations of gibberellic acid were similar for the three carbohydrases. However α-glucosidase activity increased before the other two enzymes, and a low level of α-glucosidase was found in ungerminated grain. Experiments with cycloheximide and density-labelling in deuterium oxide suggest that the observed increases in activity are the result of de novo protein synthesis. The induction of these enzymes was reduced by pre-incubation in actinomycin D. |
