Apoptosis repressor with caspase recruitment domain (ARC) inhibits myogenic differentiation |
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| Authors: | Hunter Arwen L Zhang Jingchun Chen Shirley C Si Xiaoning Wong Brian Ekhterae Daryoush Luo Honglin Granville David J |
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| Institution: | James Hogg iCAPTURE Centre for Cardiovascular and Pulmonary Research, Department of Pathology and Laboratory Medicine, St. Paul's Hospital, University of British Columbia, 1081 Burrard Street, Vancouver, BC, Canada. |
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| Abstract: | Apoptosis repressor with caspase recruitment domain (ARC), an anti-apoptotic protein, is highly expressed in differentiated heart and skeletal muscle. Apoptosis and differentiation share numerous common pathways; therefore, we examined the impact of ARC on H9c2-myoblast differentiation. We demonstrate that ARC expression levels increase and stabilize upon differentiation. ARC-overexpression in pre-differentiated H9c2-cells suppresses differentiation; indicated by increased myotube formation, nuclear fusion and expression of the differentiation markers myogenin and troponin-T. ARC-overexpression inhibited myoblast differentiation associated caspase-3 activation, suggesting ARC inhibits myogenic differentiation through caspase inhibition. In summary, we show a novel role for ARC in the regulation of muscle differentiation. |
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| Keywords: | ARC apoptosis repressor with caspase recruitment domain DMEM Dulbecco’s modified Eagle’s media FCS fetal calf serum HS horse serum RFU relative fluorescence units |
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