| 猪核糖体蛋白基因RPLP0的cDNA全长、染色体定位和多态性分析 |
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| 引用本文： | 吴潇,杨述林,朱正茂,冯书堂,李奎.猪核糖体蛋白基因RPLP0的cDNA全长、染色体定位和多态性分析[J].遗传学报,2007,34(2):104-108. |
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| 作者姓名： | 吴潇 杨述林 朱正茂 冯书堂 李奎 |
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| 作者单位： | [1]中国农业科学院畜牧研究所基因与细胞工程研究室,北京100094 [2]华中农业大学动物科技学院动物遗传育种系,华中农业大学农业动物遗传育种与繁殖教育部重点实验室,武汉430070 |
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| 基金项目： | This work was supported
by the National Natural Science Foundation of China (No.30571330),National 10th Five Year
Scientific Project of China for Tackling the Key Problems (No.2004BA717B),State Platform
of Technology Infrastructure(No.2004DKA30450),the Key Project of National Natural Science
Foundation of China (No.30330440),and Scientific Research Foundation of the State Human
Resource Ministry for Returned Chinese Scholars. |
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| 摘 要： | RPLPO基因编码酸性核糖体磷蛋白大亚基P0，是核糖体60S亚基的组成成分之一。从本室构建的猪胚胎骨骼肌cDNA文库中分离得到猪RPLPO基因的全长cDNA，并提交GenBank数据库。比较猪RPLPO基因和人及小鼠同源基因的cDNA序列和蛋白质序列，结果表明该基因在3个物种中具有高的相似性。用PCR．RFLP方法在猪RPLPO基因cDNA545处检测到C—A的单碱基突变，为Csp6Ⅰ的酶切位点。统计分析结果表明3种基因型（AA，AC，CC）在外来品种杜洛克，大约克，长白和中国地方品种通城猪，小梅山，玉山猪中的分布各不相同。同时使用体细胞杂种板（SCHP）和辐射杂种板（IMpRH）对RPLPO基因进行染色体定位，该基因被定位于SSC14q22．q24并且和SW1321微卫星标志紧密连锁（25cR，LOD=14．54）。
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| 关 键 词： | 酸性核糖体磷蛋白大亚基（RPLPO） PCR-RFLP SCHP IMpRH 遗传多态性 |
| 收稿时间： | 10 February 2006 |
| 修稿时间： | 2006-02-10 |
Characterization of the Full-length cDNA, Chromosomal Localization, and Polymorphism of the Porcine RPLPO Gene |
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| Xiao Wu,Shulin Yang, Zhengmao Zhu, Shutang Feng, Kui Li.Characterization of the Full-length cDNA, Chromosomal Localization, and Polymorphism of the Porcine RPLPO Gene[J].Journal of Genetics and Genomics,2007,34(2):104-108. |
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| Authors: | Xiao Wu Shulin Yang Zhengmao Zhu Shutang Feng Kui Li |
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| Institution: | Department of Gene and Cell Engineering, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100094, China; 2. Department of Animal Genetics and Breeding, School of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070 China |
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| Abstract: | RPLP0 gene encodes the acidic ribosomal phosphoprotein large P0 subunit, which is a component of the 60S subunit. The full-length cDNA sequence of porcine RPLP0 was obtained from skeletal muscle of fetal pig cDNA library and deposited in GenBank. The nucleotide sequence and the predicted protein sequence shared high sequence identity with other mammalian homologues. A C/A single nucleotide substitution in exon 5 was detected as Csp6?polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) shows allele frequency diversity among Tongcheng, Xiaomeishan, Yushan, Large White, Landrace, and Duroc breeds. Analyses of somatic cell hybrid panel (SCHP) and radiation hybrid (IMpRH) panel showed that the RPLP0 gene was mapped to SSC 14q22-q24 and was closely linked to locus SW1321 (25 cR, LOD = 14.54). |
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| Keywords: | RPLPO porcine IMpRH SCHP polymorphisms |
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