Quantitative determination of hemoglobin and cytochemical staining for peroxidase using 3,3',5,5'-tetramethylbenzidine dihydrochloride, a safe substitute for benzidine. |
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| Authors: | H H Liem F Cardenas M Tavassoli M B Poh-Fitzpatrick U Muller-Eberhard |
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| Institution: | 1. Department of Biochemistry, Scripps Clinic and Research Foundation, La Jolla, California 92037 USA;2. Department of Hematology, Scripps Clinic and Research Foundation, La Jolla, California 92037 USA;3. Department of Dermatology, Columbia University, College of Physicians and Surgeons, New York, New York 10032 USA |
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| Abstract: | The quantitative determination of hemoglobin employing 3,3′,5,5′-tetramethylbenzidine dihydrochloride (TMB-d) is described. This agent can also be used for staining myeloperoxidase-containing granules in granulocytes. TMB-d is at least as sensitive a reagent as benzidine but, unlike benzidine and diaminobenzidine, it is not a carcinogen. |
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